Pengaruh Fotoperiode dan Konsentrasi Sukrosa terhadap Pertumbuhan Planlet Kentang In Vitro
Keywords:
Photoperiod, Potato, Sucrose, Tissue CultureAbstract
In vitro culture is a technique of isolating the apical part or organ of a plant to be grown on an artificial medium that contains nutrients and growth regulators under aseptic conditions. This study aimed to test the main effects of photoperiod and sucrose concentration, and their interaction, on plantlet height, number of leaves, leaf color, root emergence time, and dry biomass of in vitro potato plantlets. The research was carried out from February to March 2026. The study took place at the Food Crops and Horticultural Seed Garden Laboratory, Kledung, Temanggung, Central Java. This research used a two-factor experiment with a split-plot design, followed by Tukey test. The main plot was photoperiod (0 hours/ day, 6 hours/day, 12 hours/day, 24 hours/day), and the sub-plot was sucrose concentration (0 g/L, 30 g/L, 60 g/L, 90 g/L). There were 16 treatment combinations, each repeated three times. A photoperiod of 0 hours/day resulted in the tallest plantlets, reaching 12.59 cm, but caused etiolation. Sucrose concentration influenced all the observed parameters, with 60 g/L producing the tallest plantlets at 15.66 cm. There was also an interaction between photoperiod and sucrose concentration affecting plantlet height, root emergence time, number of leaves, leaf color, number of roots, and biomass weight. The combination of a 0-hour/day photoperiod and 60 g/L sucrose produced the tallest plantlets, which was 22.52 cm.
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